magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation
function
export of magnesium
product
magnesium efflux pump
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
1,032,063 → 1,033,397
Phenotypes of a mutant
suppression of the growth defects of [gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA] and [gene|5F3933197343FBBDCBD42B408837F8EEF0A0BEBD|rplW] mutants [pubmed|29967120]partial suppression of sporulation frequency of a [gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA] mutant [pubmed|29967120]The protein
Protein family
[SW|UPF0053 family](according to UniProt)Paralogous protein(s)
[protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|YrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT] [SW|Domains]
[SW|CNNM transmembrane domain] (aa 1-201) (according to UniProt)[SW|DUF21 domain] (aa 5-201)2 [SW|CBS domain]s (aa 220-282, aa 284-344) (according to UniProt)Structure
[PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)[SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|EDFAF3AF6F2E7578733D5BF0295AD614070453F4|yhdQ]-[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]
description
[Pubmed|22383849]
regulation
threefold induced in the presence of high Mg2+ concentrations [Pubmed|31964700]view in new tabBiological materials
Mutant
MGNA-A696 (yhdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/696 NBRP B. subtilis, Japan]BKE09550 (Δ[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGTBKK09550 (Δ[gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGTExpression vectors
pGP2931 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s labReferences
10960106,22383849,25182490,27933050,29967120,31415562,31864811